武汉病毒研究所的 石正丽、葛兴义 于 2015 年 11 月 9 日在 Nature Medicine 上发表的论文，承认他们人工合成了 SARS-CoV 病毒（类SARS冠状病毒），该病毒能利用 SARS 的感染机制来感染人类。
该论文标题为：A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence
链接：https://www.nature.com/articles/nm.3985
论文作者 葛兴义、石正丽 皆来自 武汉病毒研究所：
Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
Xing-Yi Ge & Zhengli-Li Shi
论文摘要：
Using the SARS-CoV reverse genetics system2, we generated and characterized a chimeric virus expressing the spike of bat coronavirus SHC014 in a mouse-adapted SARS-CoV backbone.
The results indicate that group 2b viruses encoding the SHC014 spike in a wild-type backbone can efficiently use multiple orthologs of the SARS receptor human angiotensin converting enzyme II (ACE2), replicate efficiently in primary human airway cells and achieve in vitro titers equivalent to epidemic strains of SARS-CoV. Additionally, in vivo experiments demonstrate replication of the chimeric virus in mouse lung with notable pathogenesis.
Evaluation of available SARS-based immune-therapeutic and prophylactic modalities revealed poor efficacy; both monoclonal antibody and vaccine approaches failed to neutralize and protect from infection with CoVs using the novel spike protein.On the basis of these findings, we synthetically re-derived an infectious full-length SHC014 recombinant virus and demonstrate robust viral replication both in vitro and in vivo. Our work suggests a potential risk of SARS-CoV re-emergence from viruses currently circulating in bat populations.
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